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Catalog No: OKCD02036 (Formerly GWB-KBBTX5)
Size:96WELLS
Price: $750.00
SKU
OKCD02036
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Datasheets/ManualsPrintable datasheet for Wfs1 ELISA Kit (Mouse) (OKCD02036)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wolfram Syndrome Protein 1 (WFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wolfram Syndrome Protein 1 (WFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Wfs1 Microplate96 Wells (12 x 8 Well Strips)
Wfs1 Lyophilized Standard2 x 20 ng
100X Biotinylated Wfs1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.133 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Wolfram Syndrome Protein 1 (WFS1). No significant cross-reactivity or interference between Wolfram Syndrome Protein 1 (WFS1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolWfs1
Gene Full Namewolframin ER transmembrane glycoprotein
Alias SymbolsAI481085;wol;Wolfram syndrome 1 homolog;Wolfram syndrome 1 protein homolog;wolframin.
NCBI Gene Id22393
Protein NameWolframin
Description of TargetParticipates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store.
Uniprot IDP56695
Protein Accession #NP_035846.1
Nucleotide Accession #NM_011716.2
Protein Size (# AA)890
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