WNT16 ELISA Kit (Human) (OKCA00784)

Catalog No: OKCA00784

Size:96 Wells

Human WNT16 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	82-93
  1:2	102	97-107
  1:4	98	95-102
  1:8	93	90-96

• ELISA Kit Principle: Aviva Systems Biology WNT16 ELISA Kit (Human) (OKCA00784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT16 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WNT16 captured in the well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85	81-92
  EDTA Plasma (n=4)	100	97-103

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level WNT16 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level WNT16 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • WNT16 Microplate: 96 Wells (12 x 8 Well strips)
  • WNT16 Lyophilized Standard: 2
  • 100X Biotinylated WNT16 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma And Tissue Homogenates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: WNT16
• Gene Full Name: Wnt family member 16
• Alias Symbols: Protein Wnt-16
• NCBI Gene Id: 51384
• Protein Name: Protein Wnt-16
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Uniprot ID: Q9UBV4
• Protein Accession #: NP_057171.2
• Nucleotide Accession #: NM_016087.2