Catalog No: OKCD01835 (Formerly GWB-KBBFT2)
Size:96WELLS
Price: $675.00
SKU
OKCD01835
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Datasheets/ManualsPrintable datasheet for WASF2 ELISA Kit (Human) (OKCD01835)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-WASF2 Microplate96 Wells (12 x 8 Well Strips)
WASF2 Lyophilized Standard2 x 20 ng
100X Biotinylated WASF2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.106 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). No significant cross-reactivity or interference between Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolWASF2
Gene Full NameWASP family member 2
Alias SymbolsdJ393P12.2;IMD2;protein WAVE-2;putative Wiskott-Aldrich syndrome protein family member 4;SCAR2;suppressor of cyclic-AMP receptor (WASP-family);verprolin homology domain-containing protein 2;WAS protein family member 2;WASF4;WASP family protein member 2;WASP family protein member 4;WASP family Verprolin-homologous protein 2;WAVE2;wiskott-Aldrich syndrome protein family member 2.
NCBI Gene Id10163
Protein NameWiskott-Aldrich syndrome protein family member 2
Description of TargetDownstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Uniprot IDQ9Y6W5
Protein Accession #NP_001188333.1
Nucleotide Accession #NM_001201404.2
Protein Size (# AA)498
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