Catalog No: OKCA01007
Size:96 Wells
Price: $754.00
SKU
OKCA01007
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat103101-106
1:29693-98
1:48986-93
1:89894-103
ELISA Kit PrincipleAviva Systems Biology Trpv1 ELISA Kit (Rat) (OKCA01007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Trpv1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Trpv1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Trpv1 captured in well.
ELISA Kit Range28 pg/mL-1,800 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9288-96
EDTA Plasma (n=4)9289-97
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Trpv1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Trpv1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
Trpv1 Microplate96 Wells (12 x 8 Well strips)
Trpv1 Lyophilized Standard2
100X Biotinylated Trpv1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolTRPV1
Gene Full NameTransient receptor potential cation channel subfamily V member 1
Alias SymbolsOsm-9-like TRP channel 1, OTRPC1, Transient receptor potential cation channel subfamily V member 1, Trpv1, TrpV1, Vanilloid receptor 1
NCBI Gene Id100008926
Protein NameTransient receptor potential cation channel subfamily V member 1
Description of TargetLigand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli . Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH
Uniprot IDQ6RX08
Protein Accession #NP_001075635.1
Nucleotide Accession #NM_001082166.1
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