Catalog No: OKCD01926 (Formerly GWB-KBBCL4)
Size:96WELLS
Price: $675.00
SKU
OKCD01926
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Datasheets/ManualsPrintable datasheet for TRAF3 ELISA Kit (Human) (OKCD01926)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-97%78-93%84-102%83-97%
EDTA Plasma (n=5)78-93%89-96%82-90%78-103%
Heparin Plasma (n=5)88-97%83-102%80-93%82-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 3 (TRAF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 3 (TRAF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 3 (TRAF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNF Receptor Associated Factor 3 (TRAF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-10389
EDTA Plasma (n=5)85-9288
Heparin Plasma (n=5)86-9590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 3 (TRAF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 3 (TRAF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TRAF3 Microplate96 Wells (12 x 8 Well Strips)
TRAF3 Lyophilized Standard2 x 1 ng
100X Biotinylated TRAF3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 6.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 3 (TRAF3). No significant cross-reactivity or interference between TNF Receptor Associated Factor 3 (TRAF3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTRAF3
Gene Full NameTNF receptor associated factor 3
Alias SymbolsCAP1;CAP-1;CD40 associated protein 1;CD40 binding protein;CD40 receptor associated factor 1;CD40 receptor-associated factor 1;CD40-binding protein;CD40bp;CRAF1;IIAE5;LAP1;LMP1-associated protein 1;RING-type E3 ubiquitin transferase TRAF3;RNF118;TNF receptor-associated factor 3.
NCBI Gene Id7187
Protein NameTNF receptor-associated factor 3
Description of TargetRegulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Uniprot IDQ13114
Protein Accession #NP_001186356.1
Nucleotide Accession #NM_001199427.1
Protein Size (# AA)568
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