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Catalog No: OKCD01559 (Formerly GWB-KBBPP9)
Size:96WELLS
Price: $650.00
SKU
OKCD01559
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Datasheets/ManualsPrintable datasheet for TPPP ELISA Kit (Human) (OKCD01559)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Polymerization Promoting Protein (TPPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tubulin Polymerization Promoting Protein (TPPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Polymerization Promoting Protein (TPPP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Polymerization Promoting Protein (TPPP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TPPP Microplate96 Wells (12 x 8 Well Strips)
TPPP Lyophilized Standard2 x 20 ng
100X Biotinylated TPPP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.128 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tubulin Polymerization Promoting Protein (TPPP). No significant cross-reactivity or interference between Tubulin Polymerization Promoting Protein (TPPP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTPPP
Gene Full Nametubulin polymerization promoting protein
Alias Symbols25 kDa brain-specific protein;brain specific protein p25 alpha;glycogen synthase kinase 3 (GSK3) inhibitor p24;p24;p25;p25alpha;p25-alpha;TPPP/p25;TPPP1;tubulin polymerization-promoting protein.
NCBI Gene Id11076
Protein NameTubulin polymerization-promoting protein
Description of TargetMay play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Uniprot IDO94811
Protein Accession #NP_008961.1
Nucleotide Accession #NM_007030.2
Protein Size (# AA)219
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