TJP1 ELISA Kit (Rat) (OKCA01006)

Catalog No: OKCA01006

Size:96 Wells

Rat TJP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	91	86-95
  1:2	102	97-107
  1:4	91	85-97
  1:8	97	91-103

• ELISA Kit Principle: Aviva Systems Biology TJP1 ELISA Kit (Rat) (OKCA01006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for TJP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TJP1 captured in well.
• ELISA Kit Range: 31.2 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	89-98
  EDTA Plasma (n=4)	97	90-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level TJP1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level TJP1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • TJP1 Microplate: 96 Wells (12 x 8 Well strips)
  • TJP1 Lyophilized Standard: 2
  • 100X Biotinylated TJP1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TJP1
• Gene Full Name: Tight junction protein ZO-1
• Alias Symbols: ZO1, ZO-1
• NCBI Gene Id: 403752
• Protein Name: Tight junction protein ZO-1
• Description of Target: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
• Uniprot ID: O97758
• Protein Accession #: NP_001003140.1
• Nucleotide Accession #: NM_001003140.1