Catalog No: OKCD01938 (Formerly GWB-KBBNZ4)
Size:96WELLS
Price: $675.00
SKU
OKCD01938
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Datasheets/ManualsPrintable datasheet for TGM4 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-96%79-99%79-97%92-99%
EDTA Plasma (n=5)78-97%79-89%97-105%80-104%
Heparin Plasma (n=5)85-103%83-93%84-94%79-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 4, Prostate (TGM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 4, Prostate (TGM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 4, Prostate (TGM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 4, Prostate (TGM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-105101
EDTA Plasma (n=5)83-9793
Heparin Plasma (n=5)88-10395
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 4, Prostate (TGM4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 4, Prostate (TGM4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TGM4 Microplate96 Wells (12 x 8 Well Strips)
TGM4 Lyophilized Standard2 x 50 ng
100X Biotinylated TGM4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.33 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transglutaminase 4, Prostate (TGM4). No significant cross-reactivity or interference between Transglutaminase 4, Prostate (TGM4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTGM4
Gene Full NameTransglutaminase 4
Alias SymbolsTGP, hTGP
NCBI Gene Id7047
Protein NameProtein-glutamine gamma-glutamyltransferase 4
Description of TargetAssociated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
Uniprot IDP49221
Protein Accession #NP_003232.2
Nucleotide Accession #NM_003241.3
Protein Size (# AA)684
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