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Catalog No: OKCD02012 (Formerly GWB-KBBDJ5)
Size:96WELLS
Price: $675.00
SKU
OKCD02012
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Datasheets/ManualsPrintable datasheet for TFE3 ELISA Kit (Human) (OKCD02012)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CILP2 Microplate96 Wells (12 x 8 Well Strips)
CILP2 Lyophilized Standard2 x 20 ng
100X Biotinylated CILP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 12 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transcription Factor Binding To IGHM Enhancer 3 (TFE3). No significant cross-reactivity or interference between Transcription Factor Binding To IGHM Enhancer 3 (TFE3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTFE3
Gene Full Nametranscription factor binding to IGHM enhancer 3
Alias SymbolsbHLHe33;class E basic helix-loop-helix protein 33;RCCP2;RCCX1;TFEA;transcription factor E family, member A;transcription factor E3;transcription factor for IgH enhancer;transcription factor for immunoglobulin heavy-chain enhancer 3.
NCBI Gene Id7030
Protein NameTranscription factor E3
Description of TargetTranscription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Uniprot IDP19532
Protein Accession #NP_001269071.1
Nucleotide Accession #NM_001282142.1
Protein Size (# AA)575
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