Catalog No: OKCD02069 (Formerly GWB-KBBVU6)
Size:96WELLS
Price: $800.00
SKU
OKCD02069
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Datasheets/ManualsPrintable datasheet for Srd5a2 ELISA Kit (Rat) (OKCD02069)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-103%96-105%97-105%96-105%
EDTA Plasma (n=5)81-95%87-98%93-101%79-96%
Heparin Plasma (n=5)80-96%96-104%88-104%86-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 2 (SRD5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-10495
EDTA Plasma (n=5)84-10193
Heparin Plasma (n=5)90-10594
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Srd5a2 Microplate96 Wells (12 x 8 Well Strips)
Srd5a2 Lyophilized Standard2 x 10 ng
100X Biotinylated Srd5a2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2). No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSrd5a2
Gene Full Namesteroid 5 alpha-reductase 2
Alias Symbols3-oxo-5-alpha-steroid 4-dehydrogenase 2;5 alpha-SR2;S5AR 2;SR type 2;Steroid 5-alpha-reductase 2;steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2).
NCBI Gene Id64677
Protein Name3-oxo-5-alpha-steroid 4-dehydrogenase 2
Description of TargetConverts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids (PubMed:1527072). It plays a central role in sexual differentiation and androgen physiology (By similarity).
Uniprot IDP31214
Protein Accession #NP_073202.1
Nucleotide Accession #NM_022711.4
Protein Size (# AA)254
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