Serping1 ELISA Kit (Mouse) (OKCD01503)

Catalog No: OKCD01503 (Formerly GWB-KBBKB6)

Size:96WELLS

Mouse Serping1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	84-94%	88-99%	97-105%	78-92%
  EDTA Plasma (n=5)	85-92%	88-97%	97-105%	97-104%
  Heparin Plasma (n=5)	80-94%	80-90%	94-102%	91-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	96-103	101
  EDTA Plasma (n=5)	78-96	83
  Heparin Plasma (n=5)	84-92	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Serping1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Serping1 Lyophilized Standard: 2 x 1,000 ng
  • 100X Biotinylated Serping1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 5.9 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Serping1
• Gene Full Name: serine (or cysteine) peptidase inhibitor, clade G, member 1
• Alias Symbols: C1 esterase inhibitor;C1 Inh;C1 inhibitor;C1I;C1INH;C1-inhibiting factor;C1n;C1nh;complement component 1 inhibitor;plasma protease C1 inhibitor;serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1;serine (or cysteine) proteinase inhibitor, clade G, member 1;serpin G1.
• NCBI Gene Id: 12258
• Protein Name: Plasma protease C1 inhibitor
• Description of Target: Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
• Uniprot ID: P97290
• Protein Accession #: NP_033906.2
• Nucleotide Accession #: NM_009776.3
• Protein Size (# AA): 504