Catalog No: OKCA00923
Size:96 Wells
Price: $754.00
SKU
OKCA00923
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat8784-95
1:29591-98
1:49996-102
1:89689-103
ELISA Kit PrincipleAviva Systems Biology Sema3d ELISA Kit (Mouse) (OKCA00923) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Sema3d has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sema3d is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sema3d captured in well.
ELISA Kit Range23.5 pg/mL-1,500 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)8680-94
EDTA Plasma (n=4)9892-104
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Sema3d were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Sema3d were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
Sema3d Microplate96 Wells (12 x 8 Well strips)
Sema3d Lyophilized Standard2
100X Biotinylated Sema3d Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity5.8 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSEMA3D
Gene Full NameSema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Alias Symbols4631426B19Rik
NCBI Gene Id108151
Protein NameSemaphorin-3D
Description of TargetInduces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
Uniprot IDQ8BH34
Protein Accession #NP_083158.3
Nucleotide Accession #NM_028882.4
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