SDC4 ELISA Kit (Human) (OKCA00831)

Catalog No: OKCA00831

Size:96 Wells

Human SDC4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	90	85-95
  1:2	92	90-96
  1:4	93	85-100
  1:8	94	86-98

• ELISA Kit Principle: Aviva Systems Biology Sdc4 ELISA Kit (Human) (OKCA00831) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Sdc4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sdc4 captured in well.
• ELISA Kit Range: 0.625 ng/mL-40 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	91-99
  EDTA Plasma (n=4)	94	88-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Sdc4 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Sdc4 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Sdc4 Microplate: 96 Wells (12 x 8 Well strips)
  • Sdc4 Lyophilized Standard: 2
  • 100X Biotinylated Sdc4 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Lysates, Tissue Homogenates
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SDC4
• Gene Full Name: Syndecan 4
• Alias Symbols: S, S4, Syn, ryud, Synd4, syndec, AA959608, AW108331, ryudocan, syndecan-4
• NCBI Gene Id: 20971
• Protein Name: Syndecan-4
• Description of Target: Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
• Uniprot ID: O35988
• Protein Accession #: NP_035651.1
• Nucleotide Accession #: NM_011521.2