SCGB1A1 ELISA Kit (Human) (OKCA00636)

Catalog No: OKCA00636

Size:96 Wells

Human SCGB1A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	91	87-99
  1:2	92	85-99
  1:4	99	94-104
  1:8	100	94-105

• ELISA Kit Principle: Aviva Systems Biology SCGB1A1 ELISA Kit (Human) (OKCA00636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCGB1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCGB1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SCGB1A1 captured in the well.
• ELISA Kit Range: 6.25 ng/mL-400 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96	90-102
  EDTA Plasma (n=4)	98	93-104

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level SCGB1A1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level SCGB1A1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • SCGB1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SCGB1A1 Lyophilized Standard: 2
  • 100X Biotinylated SCGB1A1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SCGB1A1
• Gene Full Name: Secretoglobin family 1A member 1
• Alias Symbols: UGB, UP1, CC10, CC16, CCSP, UP-1, CCPBP
• NCBI Gene Id: 7356
• Protein Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Description of Target: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
• Uniprot ID: P11684
• Protein Accession #: NP_003348.1
• Nucleotide Accession #: NM_003357.4