Catalog No: OKCD02125 (Formerly GWB-KBBZU5)
Size:96WELLS
Price: $750.00
SKU
OKCD02125
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for RESP18 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-89%80-90%97-105%80-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated Endocrine Specific Protein 18 (RESP18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulated Endocrine Specific Protein 18 (RESP18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated Endocrine Specific Protein 18 (RESP18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regulated Endocrine Specific Protein 18 (RESP18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum90-10499
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated Endocrine Specific Protein 18 (RESP18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated Endocrine Specific Protein 18 (RESP18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RESP18 Microplate96 Wells (12 x 8 Well Strips)
RESP18 Lyophilized Standard2 x 5 ng
100X Biotinylated RESP18 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 27 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Regulated Endocrine Specific Protein 18 (RESP18). No significant cross-reactivity or interference between Regulated Endocrine Specific Protein 18 (RESP18) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRESP18
Gene Full NameRegulated endocrine specific protein 18
Alias SymbolsRegulated endocrine-specific protein 18
NCBI Gene Id389075
Protein NameRegulated endocrine-specific protein 18
Description of TargetMay play an important regulatory role in corticotrophs.
Uniprot IDQ5W5W9
Protein Accession #NP_001007090.3
Nucleotide Accession #NM_001007089.3
Protein Size (# AA)173
Write Your Own Review
You're reviewing:RESP18 ELISA Kit (Human) (OKCD02125)
Your Rating
We found other products you might like!