RAB5C ELISA Kit (Human) (OKCD02087)

Catalog No: OKCD02087 (Formerly GWB-KBBBK5)

Size:96WELLS

Human RAB5C ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-105%	80-97%	91-99%	83-95%
  EDTA Plasma (n=5)	80-98%	92-105%	94-102%	86-105%
  Heparin Plasma (n=5)	85-98%	81-91%	86-96%	82-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5C, Member RAS Oncogene Family (RAB5C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB5C, Member RAS Oncogene Family (RAB5C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5C, Member RAS Oncogene Family (RAB5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAB5C, Member RAS Oncogene Family (RAB5C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-95	89
  EDTA Plasma (n=5)	91-101	96
  Heparin Plasma (n=5)	87-101	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5C, Member RAS Oncogene Family (RAB5C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5C, Member RAS Oncogene Family (RAB5C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RAB5C Microplate: 96 Wells (12 x 8 Well Strips)
  • RAB5C Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated RAB5C Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAB5C, Member RAS Oncogene Family (RAB5C). No significant cross-reactivity or interference between RAB5C, Member RAS Oncogene Family (RAB5C) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RAB5C
• Gene Full Name: RAB5C, member RAS oncogene family
• Alias Symbols: RABL, L1880, RAB5L, RAB5CL
• NCBI Gene Id: 5878
• Protein Name: Ras-related protein Rab-5C
• Description of Target: Protein transport. Probably involved in vesicular traffic.
• Uniprot ID: P51148
• Protein Accession #: NP_001238968.1
• Nucleotide Accession #: NM_001252039.1
• Protein Size (# AA): 216