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Catalog No: OKCA00905
Size:96 Wells
Price: $754.00
SKU
OKCA00905
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9285-99
1:29892-103
1:49389-98
1:89187-96
ELISA Kit PrincipleAviva Systems Biology Prcp ELISA Kit (Mouse) (OKCA00905) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Prcp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prcp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Prcp captured in well.
ELISA Kit Range37.5 pg/mL-2,400 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9287-98
EDTA Plasma (n=4)9493-104
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Prcp were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Prcp were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
Prcp Microplate96 Wells (12 x 8 Well strips)
Prcp Lyophilized Standard2
100X Biotinylated Prcp Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates And Cell Lysates
Sensitivity9.37 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPRCP
Gene Full NameProlylcarboxypeptidase (angiotensinase C)
Alias SymbolsPCP, HUMPCP, AI451719, 2510048K03Rik, 2610104A14Rik
NCBI Gene Id72461
Protein NameLysosomal Pro-X carboxypeptidase
Description of TargetCleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
Uniprot IDQ7TMR0
Protein Accession #NP_082519.1
Nucleotide Accession #NM_028243.3
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