PADI4 ELISA Kit (Mouse) (OKCA00921)

Catalog No: OKCA00921

Size:96 Wells

Mouse PADI4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	91	85-99
  1:2	95	89-101
  1:4	96	92-110
  1:8	93	86-102

• ELISA Kit Principle: Aviva Systems Biology Padi4 ELISA Kit (Mouse) (OKCA00921) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Padi4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Padi4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Padi4 captured in well.
• ELISA Kit Range: 4.7 pg/mL-300 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97	88-105
  EDTA Plasma (n=4)	96	89-104

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Padi4 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Padi4 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Padi4 Microplate: 96 Wells (12 x 8 Well strips)
  • Padi4 Lyophilized Standard: 2
  • 100X Biotinylated Padi4 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 1.17 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PADI4
• Gene Full Name: Peptidyl arginine deiminase, type IV
• Alias Symbols: Pa, Pd, Pad4, Pdi4
• NCBI Gene Id: 18602
• Protein Name: Protein-arginine deiminase type-4
• Description of Target: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
• Uniprot ID: Q9Z183
• Protein Accession #: NP_035191.2
• Nucleotide Accession #: NM_011061.2