PRSS33 ELISA Kit (Human) (OKCD02115)

Catalog No: OKCD02115 (Formerly GWB-KBBDB4)

Size:96WELLS

Human PRSS33 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	79-90%	79-97%	80-101%	82-103%
  EDTA Plasma (n=5)	82-99%	86-95%	89-96%	88-95%
  Heparin Plasma (n=5)	80-96%	88-101%	87-95%	80-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 33 (PRSS33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 33 (PRSS33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 33 (PRSS33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protease, Serine 33 (PRSS33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-92	84
  EDTA Plasma (n=5)	83-92	86
  Heparin Plasma (n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease, Serine 33 (PRSS33) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease, Serine 33 (PRSS33) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PRSS33 Microplate: 96 Wells (12 x 8 Well Strips)
  • PRSS33 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PRSS33 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cell lysates and other biological fluids
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protease, Serine 33 (PRSS33). No significant cross-reactivity or interference between Protease, Serine 33 (PRSS33) and analogues was observed.
• Publications:

  Proteinase-Mediated Macrophage Signaling in Psoriatic Arthritis. Front Immunol. 11, 629726 (2021) 33763056

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for PRSS33 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: PRSS33
• Gene Full Name: serine protease 33
• Alias Symbols: EOS;protease, serine 33;serine protease 33;serine protease EOS.
• NCBI Gene Id: 260429
• Protein Name: Serine protease 33
• Description of Target: Serine protease that has amidolytic activity, cleaving its substrates before Arg residues.
• Uniprot ID: Q8NF86
• Protein Accession #: NP_690851.2
• Nucleotide Accession #: NM_152891.2
• Protein Size (# AA): 280