PRDX2 ELISA Kit (Human) (OKCA00750)

Catalog No: OKCA00750

Size:96 Wells

Human PRDX2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	85	81-95
  1:2	97	91-105
  1:4	96	92-108
  1:8	93	86-102

• ELISA Kit Principle: Aviva Systems Biology PRDX2 ELISA Kit (Human) (OKCA00750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRDX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRDX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PRDX2 captured in the well.
• ELISA Kit Range: 47 pg/mL-3,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92	89-98
  EDTA Plasma (n=4)	97	92-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level PRDX2 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level PRDX2 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • PRDX2 Microplate: 96 Wells (12 x 8 Well strips)
  • PRDX2 Lyophilized Standard: 2
  • 100X Biotinylated PRDX2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 11.75 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PRDX2
• Gene Full Name: Peroxiredoxin 2
• Alias Symbols: PRP, TSA, PRX2, PTX1, TPX1, NKEFB, PRXII, TDPX1, NKEF-B, HEL-S-2a
• NCBI Gene Id: 7001
• Protein Name: peroxiredoxin 2
• Description of Target: Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
• Uniprot ID: P32119
• Protein Accession #: NP_005800.3
• Nucleotide Accession #: NM_005809.5