PODXL ELISA Kit (Rat) (OKCA00993)

Catalog No: OKCA00993

Size:96 Wells

Rat PODXL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	81-93
  1:2	97	93-101
  1:4	89	85-93
  1:8	98	92-104

• ELISA Kit Principle: Aviva Systems Biology PODXL ELISA Kit (Rat) (OKCA00993) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for PODXL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PODXL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PODXL captured in well.
• ELISA Kit Range: 1.56 ng/mL-100 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91	85-97
  EDTA Plasma (n=4)	89	83-94

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level PODXL were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level PODXL were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • PODXL Microplate: 96 Wells (12 x 8 Well strips)
  • PODXL Lyophilized Standard: 2
  • 100X Biotinylated PODXL Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Urine, Cell Culture Supernatant
• Sensitivity: 0.39 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PODXL
• Gene Full Name: Podocalyxin
• Alias Symbols: PC, PCLP1, PCLP-1
• NCBI Gene Id: 100009552
• Protein Name: Podocalyxin
• Description of Target: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
• Uniprot ID: Q28645
• Protein Accession #: NP_001076235.1
• Nucleotide Accession #: NM_001082766.1