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Catalog No: OKCD01784 (Formerly GWB-KBBHH8)
Size:96WELLS
Price: $675.00
SKU
OKCD01784
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Datasheets/ManualsPrintable datasheet for PLCZ1 ELISA Kit (Human) (OKCD01784)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Zeta 1 (PLCz1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase C Zeta 1 (PLCz1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Zeta 1 (PLCz1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase C Zeta 1 (PLCz1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase C Zeta 1 (PLCz1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase C Zeta 1 (PLCz1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PLCZ1 Microplate96 Wells (12 x 8 Well Strips)
PLCZ1 Lyophilized Standard2 x 20 ng
100X Biotinylated PLCZ1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase C Zeta 1 (PLCz1). No significant cross-reactivity or interference between Phospholipase C Zeta 1 (PLCz1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPLCZ1
Gene Full Namephospholipase C zeta 1
Alias Symbols1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1;NYD-SP27;phosphoinositide phospholipase C-zeta-1;Phospholipase C-zeta-1;PI-phospholipase C zeta 1;PLCzeta;PLC-zeta-1;SPGF17;testicular tissue protein Li 145;Testis-development protein NYD-SP27;testis-development related NYD-SP27.
NCBI Gene Id89869
Protein Name1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Description of TargetThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Uniprot IDQ86YW0
Protein Accession #NP_149114.2
Nucleotide Accession #NM_033123.3
Protein Size (# AA)608
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