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Catalog No: OKCD01526 (Formerly GWB-KBBIO1)
Size:96WELLS
Price: $650.00
SKU
OKCD01526
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Datasheets/ManualsPrintable datasheet for PLAA ELISA Kit (Human) (OKCD01526)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-104%89-101%97-105%85-94%
EDTA Plasma (n=5)88-102%79-104%79-97%82-102%
Heparin Plasma (n=5)80-94%78-102%86-103%85-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Activating Protein (PLAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2 Activating Protein (PLAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2 Activating Protein (PLAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase A2 Activating Protein (PLAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)99-105102
EDTA Plasma (n=5)84-9791
Heparin Plasma (n=5)81-10392
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2 Activating Protein (PLAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2 Activating Protein (PLAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PLAA Microplate96 Wells (12 x 8 Well Strips)
PLAA Lyophilized Standard2 x 20 ng
100X Biotinylated PLAA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.105 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2 Activating Protein (PLAA). No significant cross-reactivity or interference between Phospholipase A2 Activating Protein (PLAA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPLAA
Gene Full Namephospholipase A2 activating protein
Alias SymbolsDOA1;DOA1 homolog;NDMSBA;phospholipase A-2-activating protein;PLA2P;PLAP.
NCBI Gene Id9373
Protein NamePhospholipase A-2-activating protein
Description of TargetPlays a role in protein ubiquitination, sorting and degradation through its association with VCP (PubMed:27753622). Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling (By similarity). May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed:27753622). Plays a role in cerebellar Purkinje cell development (By similarity). Positively regulates cytosolic and calcium-independent phospholipase A2 activities in a tumor necrosis factor alpha (TNF-alpha)- or lipopolysaccharide (LPS)-dependent manner, and hence prostaglandin E2 biosynthesis (PubMed:18291623, PubMed:28007986).
Uniprot IDQ9Y263
Protein Accession #NP_001026859.1
Nucleotide Accession #NM_001031689.2
Protein Size (# AA)795
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