PICALM ELISA Kit (Human) (OKCD01896)

Catalog No: OKCD01896 (Formerly GWB-KBBXL1)

Size:96WELLS

Human PICALM ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PICALM Microplate: 96 Wells (12 x 8 Well Strips)
  • PICALM Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PICALM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM). No significant cross-reactivity or interference between Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PICALM
• Gene Full Name: phosphatidylinositol binding clathrin assembly protein
• Alias Symbols: CALM;clathrin assembly lymphoid myeloid leukemia protein;CLTH;LAP;phosphatidylinositol-binding clathrin assembly protein.
• NCBI Gene Id: 8301
• Protein Name: Phosphatidylinositol-binding clathrin assembly protein
• Description of Target: Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
• Uniprot ID: Q13492
• Protein Accession #: NP_001008660.1
• Nucleotide Accession #: NM_001008660.2
• Protein Size (# AA): 652