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Catalog No: OKCD01589 (Formerly GWB-KBBXK8)
Size:96WELLS
Price: $650.00
SKU
OKCD01589
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Datasheets/ManualsPrintable datasheet for PDIA2 ELISA Kit (Human) (OKCD01589)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase (PDI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase (PDI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase (PDI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Disulfide Isomerase (PDI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PDIA2 Microplate96 Wells (12 x 8 Well Strips)
PDIA2 Lyophilized Standard2 x 20 ng
100X Biotinylated PDIA2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A2 (PDIA2). No significant cross-reactivity or interference between Protein Disulfide Isomerase A2 (PDIA2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPDIA2
Gene Full Nameprotein disulfide isomerase family A member 2
Alias Symbolspancreas-specific protein disulfide isomerase;pancreatic protein disulfide isomerase;PDA2;PDI;PDIP;PDIR;protein disulfide isomerase, pancreatic;protein disulfide isomerase-associated 2;protein disulfide-isomerase A2;Rho GDP dissociation inhibitor gamma.
NCBI Gene Id64714
Protein NameProtein disulfide-isomerase A2
Description of TargetActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Uniprot IDQ13087
Protein Accession #NP_006840.2
Nucleotide Accession #NM_006849.2
Protein Size (# AA)525
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