PAWR ELISA Kit (Human) (OKCA00758)

Catalog No: OKCA00758

Size:96 Wells

Human PAWR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	85	80-91
  1:2	90	84-98
  1:4	92	86-100
  1:8	95	88-101

• ELISA Kit Principle: Aviva Systems Biology PAWR ELISA Kit (Human) (OKCA00758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PAWR has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PAWR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PAWR captured in the well.
• ELISA Kit Range: 25 pg/mL-1,600 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97	90-102
  EDTA Plasma (n=4)	94	90-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level PAWR were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level PAWR were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • PAWR Microplate: 96 Wells (12 x 8 Well strips)
  • PAWR Lyophilized Standard: 2
  • 100X Biotinylated PAWR Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 6.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PAWR
• Gene Full Name: Pro-apoptotic WT1 regulator
• Alias Symbols: PAR4, Par-4
• NCBI Gene Id: 5074
• Protein Name: PRKC apoptosis WT1 regulator protein
• Description of Target: Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
• Uniprot ID: Q96IZ0
• Protein Accession #: NP_002574.2
• Nucleotide Accession #: NM_002583.2