PALMD ELISA Kit (Human) (OKCD01955)

Catalog No: OKCD01955 (Formerly GWB-KBBNG8)

Size:96WELLS

Human PALMD ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Palmdelphin (PALMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Palmdelphin (PALMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Palmdelphin (PALMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Palmdelphin (PALMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Palmdelphin (PALMD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Palmdelphin (PALMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PALMD Microplate: 96 Wells (12 x 8 Well Strips)
  • PALMD Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated PALMD Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.61 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Palmdelphin (PALMD). No significant cross-reactivity or interference between Palmdelphin (PALMD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PALMD
• Gene Full Name: Palmdelphin
• Alias Symbols: PALML, C1orf11
• NCBI Gene Id: 54873
• Protein Name: Palmdelphin
• Description of Target: This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q9NP74
• Protein Accession #: NP_060204.1
• Nucleotide Accession #: NM_017734.4
• Protein Size (# AA): 551