PADI4 ELISA Kit (Human) (OKCD01879)

Catalog No: OKCD01879 (Formerly GWB-KBBTK1)

Size:96WELLS

Human PADI4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	97-104%	85-94%	83-96%	90-104%
  EDTA Plasma (n=5)	85-102%	78-98%	98-105%	91-99%
  Heparin Plasma (n=5)	98-105%	88-103%	78-97%	78-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type IV (PADI4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidyl Arginine Deiminase Type IV (PADI4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	82-94	89
  EDTA Plasma (n=5)	78-94	89
  Heparin Plasma (n=5)	86-95	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PADI4 Microplate: 96 Wells (12 x 8 Well Strips)
  • PADI4 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PADI4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.121 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type IV (PADI4). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type IV (PADI4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PADI4
• Gene Full Name: peptidyl arginine deiminase 4
• Alias Symbols: HL-60 PAD;PAD;PAD4;PADI5;PADI-H protein;PDI4;PDI5;peptidyl arginine deiminase, type IV;peptidyl arginine deiminase, type V;Peptidylarginine deiminase IV;protein-arginine deiminase type IV;protein-arginine deiminase type-4.
• NCBI Gene Id: 23569
• Protein Name: Protein-arginine deiminase type-4
• Description of Target: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
• Uniprot ID: Q9UM07
• Protein Accession #: NP_036519.2
• Nucleotide Accession #: NM_012387.2.
• Protein Size (# AA): 663