NPPB ELISA Kit (Dog) (OKCA00583)

Catalog No: OKCA00583

Size:96 Wells

Dog NPPB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	95	90-105
  1:2	91	88-96
  1:4	95	90-102
  1:8	97	92-103

• ELISA Kit Principle: Aviva Systems Biology NPPB ELISA Kit (Dog) (OKCA00583) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPPB captured in the well.
• ELISA Kit Range: 12.5 pg/mL-800 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98	93-105
  EDTA Plasma (n=4)	96	92-101

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level GAST were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level GAST were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • NPPB Microplate: 96 Wells (12 x 8 Well strips)
  • NPPB Lyophilized Standard: 2
  • 100X Biotinylated NPPB Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.12 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPPB
• Gene Full Name: Natriuretic peptides B
• Alias Symbols: BNP, Iso-ANP
• NCBI Gene Id: 493764
• Protein Name: Natriuretic peptides B
• Description of Target: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
• Uniprot ID: Q9GLK4
• Protein Accession #: NP_001009244.1
• Nucleotide Accession #: NM_001009244.1