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Catalog No: OKCD02031 (Formerly GWB-KBBUA4)
Size:96WELLS
Price: $675.00
SKU
OKCD02031
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Datasheets/ManualsPrintable datasheet for NOD2 ELISA Kit (Human) (OKCD02031)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NOD2 Microplate96 Wells (12 x 8 Well Strips)
NOD2 Lyophilized Standard2 x 10 ng
100X Biotinylated NOD2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). No significant cross-reactivity or interference between Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNOD2
Gene Full Namenucleotide binding oligomerization domain containing 2
Alias SymbolsACUG;BLAU;BLAUS;CARD15;caspase recruitment domain family, member 15;caspase recruitment domain protein 15;caspase recruitment domain-containing protein 15;CD;CLR16.3;IBD1;inflammatory bowel disease protein 1;NLR family, CARD domain containing 2;NLRC2;NOD2B;NOD-like receptor C2;nucleotide-binding oligomerization domain 2;nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 2;nucleotide-binding oligomerization domain-containing protein 2;PSORAS1;YAOS.
NCBI Gene Id64127
Protein NameNucleotide-binding oligomerization domain-containing protein 2
Description of TargetInvolved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1 (PubMed:20637199). Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon (PubMed:19701189).
Uniprot IDQ9HC29
Protein Accession #NP_001280486.1
Nucleotide Accession #NM_001293557.1
Protein Size (# AA)1040
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