NET1 ELISA Kit (Human) (OKCD01961)

Catalog No: OKCD01961 (Formerly GWB-KBBMS9)

Size:96WELLS

Human NET1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroepithelial Cell Transforming Gene 1 (NET1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuroepithelial Cell Transforming Gene 1 (NET1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroepithelial Cell Transforming Gene 1 (NET1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroepithelial Cell Transforming Gene 1 (NET1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NET1 Microplate: 96 Wells (12 x 8 Well Strips)
  • NET1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated NET1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuroepithelial Cell Transforming Gene 1 (NET1). No significant cross-reactivity or interference between Neuroepithelial Cell Transforming Gene 1 (NET1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NET1
• Gene Full Name: Neuroepithelial cell transforming 1
• Alias Symbols: NET1A, ARHGEF8
• NCBI Gene Id: 10276
• Protein Name: Neuroepithelial cell-transforming gene 1 protein
• Description of Target: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
• Uniprot ID: Q7Z628
• Protein Accession #: NP_001040625.1
• Nucleotide Accession #: NM_001047160.2
• Protein Size (# AA): 596