Catalog No: OKCD02119 (Formerly GWB-KBBHR1)
Size:96WELLS
Price: $750.00
SKU
OKCD02119
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Datasheets/ManualsPrintable datasheet for N6AMT1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-N6AMT1 Microplate96 Wells (12 x 8 Well Strips)
N6AMT1 Lyophilized Standard2 x 40 ng
100X Biotinylated N6AMT1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.264 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1). No significant cross-reactivity or interference between N-6-Adenine Specific DNA Methyltransferase 1 (N6AMT1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolN6AMT1
Gene Full NameN-6 adenine-specific DNA methyltransferase 1 (putative)
Alias SymbolsKMT9, MTQ2, PrmC, HEMK2, N6AMT, PRED28, C21orf127, m.HsaHemK2P
NCBI Gene Id29104
Protein NameHemK methyltransferase family member 2
Description of TargetHeterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid.
Uniprot IDQ9Y5N5
Protein Accession #NP_037372.4
Nucleotide Accession #NM_013240.5
Protein Size (# AA)214
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