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Catalog No: OKCD01963 (Formerly GWB-KBBFL8)
Size:96WELLS
Price: $675.00
SKU
OKCD01963
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Datasheets/ManualsPrintable datasheet for MYF5 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myogenic Factor 5 (MYF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myogenic Factor 5 (MYF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myogenic Factor 5 (MYF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myogenic Factor 5 (MYF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myogenic Factor 5 (MYF5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myogenic Factor 5 (MYF5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MYF5 Microplate96 Wells (12 x 8 Well Strips)
MYF5 Lyophilized Standard2 x 10 ng
100X Biotinylated MYF5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.066 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myogenic Factor 5 (MYF5). No significant cross-reactivity or interference between Myogenic Factor 5 (MYF5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMYF5
Gene Full NameMyogenic factor 5
Alias SymbolsEORVA, bHLHc2
NCBI Gene Id4617
Protein NameMyogenic factor 5
Description of TargetActs as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Uniprot IDP13349
Protein Accession #NP_005584.2
Nucleotide Accession #NM_005593.2
Protein Size (# AA)255
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