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Catalog No: OKCD01964 (Formerly GWB-KBBFW3)
Size:96WELLS
Price: $675.00
SKU
OKCD01964
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Datasheets/ManualsPrintable datasheet for MVD ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mevalonate Decarboxylase (MVD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mevalonate Decarboxylase (MVD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mevalonate Decarboxylase (MVD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mevalonate Decarboxylase (MVD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mevalonate Decarboxylase (MVD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mevalonate Decarboxylase (MVD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MVD Microplate96 Wells (12 x 8 Well Strips)
MVD Lyophilized Standard2 x 100 ng
100X Biotinylated MVD Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.55 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mevalonate Decarboxylase (MVD). No significant cross-reactivity or interference between Mevalonate Decarboxylase (MVD) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMVD
Gene Full NameMevalonate diphosphate decarboxylase
Alias SymbolsMPD, MDDase, POROK7, FP17780
NCBI Gene Id4597
Protein NameDiphosphomevalonate decarboxylase
Description of TargetPerforms the first committed step in the biosynthesis of isoprenes.
Uniprot IDP53602
Protein Accession #NP_002452.1
Nucleotide Accession #NM_002461.2
Protein Size (# AA)400
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