MT-RNR2 ELISA Kit (Human) (OKCA00791)

Catalog No: OKCA00791

Size:96 Wells

Human MT-RNR2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:5	100	92-105
  1:10	97	91-105
  1:20	97	92-106
  1:40	93	86-98

• ELISA Kit Principle: Aviva Systems Biology MT-RNR2 ELISA Kit (Human) (OKCA00791) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MT-RNR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT-RNR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MT-RNR2 captured in the well.
• ELISA Kit Range: 28 pg/mL-1,800 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92	85-99
  EDTA Plasma (n=4)	95	90-101

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level MT-RNR2 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level MT-RNR2 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • MT-RNR2 Microplate: 96 Wells (12 x 8 Well strips)
  • MT-RNR2 Lyophilized Standard: 2
  • 100X Biotinylated MT-RNR2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MT-RNR2
• Gene Full Name: Humanin
• Protein Name: Humanin
• Description of Target: Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.
• Uniprot ID: Q8IVG9