MMP3 ELISA Kit (Horse) (OKCD01474)

Catalog No: OKCD01474 (Formerly GWB-KBBEP5)

Size:96WELLS

Horse MMP3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Equus caballus|Horse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-93%	96-104%	88-101%	85-93%
  Heparin Plasma (n=5)	78-104%	90-98%	80-91%	96-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3 (MMP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 3 (MMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 3 (MMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 3 (MMP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	85-99	92
  Heparin Plasma (n=5)	85-92	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MMP3 Microplate: 96 Wells (12 x 8 Well Strips)
  • MMP3 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated MMP3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 12.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3). No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MMP3
• Gene Full Name: matrix metallopeptidase 3
• Alias Symbols: matrix metallopeptidase 3 (stromelysin 1, progelatinase);matrix metalloproteinase 3;Matrix metalloproteinase-3;MMP-3;SL-1;stromelysin-1.
• NCBI Gene Id: 100034195
• Protein Name: Stromelysin-1
• Description of Target: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
• Uniprot ID: Q28397
• Protein Accession #: NP_001075964.1
• Nucleotide Accession #: NM_001082495.2
• Protein Size (# AA): 477