LIPH ELISA Kit (Human) (OKCD01809)

Catalog No: OKCD01809 (Formerly GWB-KBBFL5)

Size:96WELLS

Human LIPH ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	96-103%	79-96%	81-101%	86-103%
  EDTA Plasma (n=5)	84-93%	78-104%	93-101%	90-97%
  Heparin Plasma (n=5)	85-101%	78-101%	79-95%	80-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase H (LIPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase H (LIPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase H (LIPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase H (LIPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	95-104	98
  EDTA Plasma (n=5)	85-97	91
  Heparin Plasma (n=5)	90-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase H (LIPH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase H (LIPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LIPH Microplate: 96 Wells (12 x 8 Well Strips)
  • LIPH Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated LIPH Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.68 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase H (LIPH). No significant cross-reactivity or interference between Lipase H (LIPH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LIPH
• Gene Full Name: Lipase H
• Alias Symbols: AH, LAH2, ARWH2, HYPT7, LPDLR, PLA1B, mPA-PLA1
• NCBI Gene Id: 200879
• Protein Name: Lipase member H
• Description of Target: Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
• Uniprot ID: Q8WWY8
• Protein Accession #: NP_640341.1
• Nucleotide Accession #: NM_139248.2
• Protein Size (# AA): 451