LIPA ELISA Kit (Human) (OKCA00718)

Catalog No: OKCA00718

Size:96 Wells

Human LIPA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	82-90
  1:2	105	101-09
  1:4	88	85-91
  1:8	94	91-99

• ELISA Kit Principle: Aviva Systems Biology LIPA ELISA Kit (Human) (OKCA00718) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LIPA captured in the well.
• ELISA Kit Range: 62.5 pg/mL-4,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86	82-90
  EDTA Plasma (n=4)	94	90-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level LIPA were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level LIPA were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • LIPA Microplate: 96 Wells (12 x 8 Well strips)
  • LIPA Lyophilized Standard: 2
  • 100X Biotinylated LIPA Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates And Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LIPA
• Gene Full Name: Lipase A, lysosomal acid type
• Alias Symbols: LAL, CESD
• NCBI Gene Id: 3988
• Protein Name: Lysosomal acid lipase/cholesteryl ester hydrolase
• Description of Target: Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.
• Uniprot ID: P38571
• Protein Accession #: NP_000226.2
• Nucleotide Accession #: NM_000235.3