LDHA ELISA Kit (Rat) (OKCA00980)

Catalog No: OKCA00980

Size:96 Wells

Rat LDHA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	92	80-97
  1:2	92	88-105
  1:4	97	92-104
  1:8	94	86-98

• ELISA Kit Principle: Aviva Systems Biology LDHA ELISA Kit (Rat) (OKCA00980) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for LDHA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LDHA captured in well.
• ELISA Kit Range: 39 mU/mL-2,500 mU/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93	89-100
  EDTA Plasma (n=4)	90	85-95

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level LDHA were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level LDHA were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • LDHA Microplate: 96 Wells (12 x 8 Well strips)
  • LDHA Lyophilized Standard: 2
  • 100X Biotinylated LDHA Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 9.77mU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LDHA
• Gene Full Name: L-lactate dehydrogenase A chain
• Alias Symbols: LDH-M
• NCBI Gene Id: 100009107
• Protein Name: L-lactate dehydrogenase A chain
• Description of Target: This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
• Uniprot ID: P13491
• Protein Accession #: NP_001075746.1
• Nucleotide Accession #: NM_001082277.1