Klf1 ELISA Kit (Mouse) (OKCD01757)

Catalog No: OKCD01757 (Formerly GWB-KBBSD4)

Size:96WELLS

Mouse Klf1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-105%	83-90%	83-90%	79-89%
  EDTA Plasma (n=5)	83-98%	91-101%	90-97%	82-101%
  Heparin Plasma (n=5)	80-94%	87-95%	80-105%	87-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 1, Erythroid (KLF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 1, Erythroid (KLF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 1, Erythroid (KLF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kruppel Like Factor 1, Erythroid (KLF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-98	93
  EDTA Plasma (n=5)	81-102	97
  Heparin Plasma (n=5)	86-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kruppel Like Factor 1, Erythroid (KLF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kruppel Like Factor 1, Erythroid (KLF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Klf1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Klf1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Klf1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 5.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kruppel Like Factor 1, Erythroid (KLF1). No significant cross-reactivity or interference between Kruppel Like Factor 1, Erythroid (KLF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KLF1
• Gene Full Name: Kruppel-like factor 1 (erythroid)
• Alias Symbols: Ekl, Nan, Eklf
• NCBI Gene Id: 16596
• Protein Name: Krueppel-like factor 1
• Description of Target: Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
• Uniprot ID: P46099
• Protein Accession #: NP_034765.2
• Nucleotide Accession #: NM_010635.2
• Protein Size (# AA): 358