KIR3DL1 ELISA Kit (Human) (OKCD01591)

Catalog No: OKCD01591 (Formerly GWB-KBBJI3)

Size:96WELLS

Human KIR3DL1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KIR3DL1 Microplate: 96 Wells (12 x 8 Well Strips)
  • KIR3DL1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated KIR3DL1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). No significant cross-reactivity or interference between Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KIR3DL1
• Gene Full Name: killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1
• Alias Symbols: CD158 antigen-like family member E;CD158E1;HLA-BW4-specific inhibitory NK cell receptor;killer cell immunoglobulin-like receptor 3DL1;killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1;KIR;KIR antigen 3DL1;KIR2DL5B;KIR3DL1/S1;MHC class I NK cell receptor;natural killer-associated transcript 3;NKAT3;NKAT-3;NKB1;NKB1B;p70 killer cell inhibitory receptor;p70 natural killer cell receptor clones CL-2/CL-11;p70 NK receptor CL-2/CL-11.
• NCBI Gene Id: 3811
• Protein Name: Killer cell immunoglobulin-like receptor 3DL1
• Description of Target: Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
• Uniprot ID: P43629
• Protein Accession #: NP_001309097.1
• Nucleotide Accession #: NM_001322168.1
• Protein Size (# AA): 444