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Catalog No: OKCD01786 (Formerly GWB-KBBVM2)
Size:96WELLS
Price: $700.00
SKU
OKCD01786
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Datasheets/ManualsPrintable datasheet for INSL6 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)92-99%98-105%80-91%90-103%
EDTA Plasma (n=5)99-105%99-105%81-89%85-102%
Heparin Plasma (n=5)94-105%79-97%89-98%89-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Protein 6 (INSL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Protein 6 (INSL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Protein 6 (INSL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Protein 6 (INSL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)93-10297
EDTA Plasma (n=5)87-9690
Heparin Plasma (n=5)92-9995
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Protein 6 (INSL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Protein 6 (INSL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Insl6 Microplate96 Wells (12 x 8 Well Strips)
Insl6 Lyophilized Standard2 x 5 ng
100X Biotinylated Insl6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 29 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Protein 6 (INSL6). No significant cross-reactivity or interference between Insulin Like Protein 6 (INSL6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolINSL6
Gene Full NameInsulin-like 6
Alias SymbolsRI, RIF1
NCBI Gene Id27356
Protein NameInsulin-like peptide INSL6
Description of TargetMay have a role in sperm development and fertilization.
Uniprot IDQ9QY05
Protein Accession #NP_038782.1
Nucleotide Accession #NM_013754.1
Protein Size (# AA)191
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