IL12A ELISA Kit (Goat) (OKCD01451)

Catalog No: OKCD01451 (Formerly GWB-KBBQH7)

Size:96WELLS

Goat IL12A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Capra hircus|Goat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	91-105%	79-94%	88-101%	92-99%
  EDTA Plasma (n=5)	81-102%	87-94%	78-102%	87-95%
  Heparin Plasma (n=5)	90-99%	90-103%	79-91%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12A (IL12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12A (IL12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12A (IL12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 12A (IL12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-96	92
  EDTA Plasma (n=5)	86-101	94
  Heparin Plasma (n=5)	92-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL12A Microplate: 96 Wells (12 x 8 Well Strips)
  • IL12A Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated IL12A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 12A (IL12A). No significant cross-reactivity or interference between Interleukin 12A (IL12A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL12A
• Gene Full Name: interleukin 12A
• Alias Symbols: CLMF p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;IL-12 subunit p35;IL-12A;interleukin 12 35kD subunit;interleukin 12 p35 subunit;interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35);interleukin-12 subunit alpha.
• NCBI Gene Id: 100861293
• Protein Name: Interleukin-12 subunit alpha
• Description of Target: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
• Uniprot ID: O02814
• Protein Accession #: NP_001272567.1
• Nucleotide Accession #: NM_001285638.1
• Protein Size (# AA): 221