Catalog No: OKCD01906 (Formerly GWB-KBBNR7)
Size:96 Wells
Price: $675.00
SKU
OKCD01906
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-103%92-101%80-89%97-104%
ELISA Kit PrincipleAviva Systems Biology IER3 ELISA Kit (Human) (OKCD01906) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IER3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IER3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IER3 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum97-105101
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level IER3 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level IER3 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-IER3 Microplate96 Wells (12 x 8 Well Strips)
IER3 Lyophilized Standard2 x 10 ng
100X Biotinylated IER3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Immediate Early Response 3 (IER3). No significant cross-reactivity or interference between Immediate Early Response 3 (IER3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIER3
Gene Full NameImmediate early response 3
Alias SymbolsDIF2, IEX1, PRG1, DIF-2, GLY96, IEX-1, IEX-1L
NCBI Gene Id8870
Protein NameRadiation-inducible immediate-early gene IEX-1
Description of TargetMay play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
Uniprot IDP46695
Protein Accession #NP_003888.2
Nucleotide Accession #NM_003897.3
Protein Size (# AA)156
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