IBA57 ELISA Kit (Human) (OKCD02122)

Catalog No: OKCD02122 (Formerly GWB-KBBXS4)

Size:96WELLS

Human IBA57 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IBA57 Microplate: 96 Wells (12 x 8 Well Strips)
  • IBA57 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated IBA57 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57). No significant cross-reactivity or interference between IBA57, Iron Sulfur Cluster Assembly Factor Homolog (IBA57) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IBA57
• Gene Full Name: IBA57 homolog, iron-sulfur cluster assembly
• Alias Symbols: MMDS3, SPG74, C1orf69
• NCBI Gene Id: 200205
• Protein Name: Putative transferase CAF17, mitochondrial
• Description of Target: Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.
• Uniprot ID: Q5T440
• Protein Accession #: NP_001010867.1
• Nucleotide Accession #: NM_001010867.3
• Protein Size (# AA): 356