HYOU1 ELISA Kit (Human) (OKCD01680)

Catalog No: OKCD01680 (Formerly GWB-KBBUY8)

Size:96WELLS

Human HYOU1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-101%	82-89%	78-95%	87-101%
  EDTA Plasma (n=5)	81-94%	82-98%	91-102%	78-101%
  Heparin Plasma (n=5)	78-89%	95-103%	81-91%	93-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 150 kDa Oxygen Regulated Protein (ORP150). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 150 kDa Oxygen Regulated Protein (ORP150). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 150 kDa Oxygen Regulated Protein (ORP150), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 150 kDa Oxygen Regulated Protein (ORP150) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	87-96	90
  EDTA Plasma (n=5)	78-97	82
  Heparin Plasma (n=5)	95-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 150 kDa Oxygen Regulated Protein (ORP150) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 150 kDa Oxygen Regulated Protein (ORP150) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HYOU1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HYOU1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HYOU1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hypoxia Up Regulated 1 (HYOU1). No significant cross-reactivity or interference between Hypoxia Up Regulated 1 (HYOU1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HYOU1
• Gene Full Name: hypoxia up-regulated 1
• Alias Symbols: 150 kDa oxygen-regulated protein;170 kDa glucose-regulated protein;epididymis secretory sperm binding protein;Grp170;GRP-170;HSP12A;hypoxia up-regulated protein 1;IMD59;ORP150;ORP-150;oxygen regulated protein (150kD).
• NCBI Gene Id: 10525
• Protein Name: Hypoxia up-regulated protein 1
• Description of Target: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
• Uniprot ID: Q9Y4L1
• Protein Accession #: NP_001124463.1
• Nucleotide Accession #: NM_001130991.2
• Protein Size (# AA): 461