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Catalog No: OKCA00977
Size:96 Wells
Price: $929.00
SKU
OKCA00977
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9287-97
1:29689-103
1:410298-106
1:88781-95
ELISA Kit PrincipleAviva Systems Biology HSP90AA1 ELISA Kit (Rat) (OKCA00977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for HSP90AA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSP90AA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HSP90AA1 captured in well.
ELISA Kit Range0.312 ng/mL-20 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9087-94
EDTA Plasma (n=4)10197-105
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level HSP90AA1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level HSP90AA1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
HSP90AA1 Microplate96 Wells (12 x 8 Well strips)
HSP90AA1 Lyophilized Standard2
100X Biotinylated HSP90AA1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolHSP90AA1
Gene Full NameHeat shock protein HSP 90-alpha
Protein NameHeat shock protein HSP 90-alpha
Description of TargetMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Uniprot IDP30946
Protein Accession #XP_002721768.1
Nucleotide Accession #XM_002721722.3
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