Catalog No: OKCD01678 (Formerly GWB-KBBSV6)
Size:96WELLS
Price: $675.00
SKU
OKCD01678
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Datasheets/ManualsPrintable datasheet for HHIP ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Interacting Protein (HHIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Interacting Protein (HHIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Interacting Protein (HHIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hedgehog Interacting Protein (HHIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Interacting Protein (HHIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Interacting Protein (HHIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HHIP Microplate96 Wells (12 x 8 Well Strips)
HHIP Lyophilized Standard2 x 20 ng
100X Biotinylated HHIP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.107 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hedgehog Interacting Protein (HHIP). No significant cross-reactivity or interference between Hedgehog Interacting Protein (HHIP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHHIP
Gene Full NameHedgehog interacting protein
Alias SymbolsHIP
NCBI Gene Id64399
Protein NameHedgehog-interacting protein
Description of TargetModulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.
Uniprot IDQ96QV1
Protein Accession #NP_071920.1
Nucleotide Accession #NM_022475.2
Protein Size (# AA)700
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