HAT1 ELISA Kit (Human) (OKCD01617)

Catalog No: OKCD01617 (Formerly GWB-KBBBV5)

Size:96WELLS

Human HAT1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Acetyltransferase 1 (HAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Acetyltransferase 1 (HAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Acetyltransferase 1 (HAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Acetyltransferase 1 (HAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Acetyltransferase 1 (HAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Acetyltransferase 1 (HAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HAT1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HAT1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated HAT1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.103 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Acetyltransferase 1 (HAT1). No significant cross-reactivity or interference between Histone Acetyltransferase 1 (HAT1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HAT1
• Gene Full Name: Histone acetyltransferase 1
• Alias Symbols: KAT1
• NCBI Gene Id: 8520
• Protein Name: Histone acetyltransferase type B catalytic subunit
• Description of Target: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
• Uniprot ID: O14929
• Protein Accession #: NP_003633.1
• Nucleotide Accession #: NM_003642.3
• Protein Size (# AA): 419