Catalog No: OKCD01870 (Formerly GWB-KBBGT2)
Size:96WELLS
Price: $675.00
SKU
OKCD01870
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Datasheets/ManualsPrintable datasheet for HAND2 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-98%87-99%91-104%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum83-10190
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HAND2 Microplate96 Wells (12 x 8 Well Strips)
HAND2 Lyophilized Standard2 x 10 ng
100X Biotinylated HAND2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2). No significant cross-reactivity or interference between Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHAND2
Gene Full NameHeart and neural crest derivatives expressed 2
Alias SymbolsHed, dHand, DHAND2, Thing2, bHLHa26
NCBI Gene Id9464
Protein NameHeart- and neural crest derivatives-expressed protein 2
Description of TargetEssential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
Uniprot IDP61296
Protein Accession #NP_068808.1
Nucleotide Accession #NM_021973.2
Protein Size (# AA)217
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